Instrument settings are listed in Table 1. In addition, SoftMax Pro Software Molecular Devices SpectraMax ® Paradigm ® Multi-Mode Microplate Detection Platform is a user upgradeable microplate reader that allows for real-time system configuration by the user in less than two minutes. 00. HepG2 cells were plated into a 96-well clear-bottom, white-walled plate at 10,000 cells/well and incubated at 37°C overnight. 0200-6262. Comparing Luminescence sensitivity of SpectraMax Microplate Readers using ATP Assay | Molecular Devices Author: Molecular DevicesMake the most of your budget with the VersaMax™ ELISA Microplate Reader, our affordable, visible spectrum absorbance reader for 96-well microplates. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. With various on-board features and. Standard SBS 96 and 384 well plates (128. Endpoint, kinetic, spectrum. 69: 85. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. Key applications: ELISA, nucleic acid, endotoxin testing. A multitude of modalities. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. LVF Monochromator™ : Excitation and Emission. I contacted Molecular Devices and they quoted me almost $6k to come out and replace. The IC design and microplate construct represents a major advancement to state-of-the-art CMOS devices for biological measurement, where the large majority are. This reader's dual monochromators for. The SpectraDrop Microplate’s slide design eliminates the need for calibration and gives consistent well-to-well reads with CVs below 5%. 4. #12-639) White 96- and 384-well microplates (Greiner cat. Microplate readers compatible with 96-well plates Single- to multi-mode readers combine an absorbance plate reader , a luminescence plate reader , and a fluorescence plate. For more sample throughput, the system can be. The CellTiter-Glo assay from Promega uses luciferase. 0 500 1000 1500 2000 2500 3000 3500 time (sec. ; Page 2 Molecular Devices Corporation warrants this product against defects in material or workmanship as follows:. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Important things to consider when purchasing a multimode microplate reader. Reactions catalyzed by firefly and *Renilla luciferases. Compact design Minimum volume and maximum throughput with 96- or 384-well microplate compatibility Powerful data analysis with SoftMax® Pro Software Optimal performance with validation tools Small but Mighty The IC design and microplate construct represents a major advancement to state-of-the-art CMOS devices for biological measurement, where the large majority are formed in single well constructs 12. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. emax and Vmax microplate readers. Place a lid on the microplate and put the plate in the drawer of the reader and mix for approximately 5 seconds. 96-well plates: V-bottom: 20-30 µl U-bottom: 30-40 µl flat-bottom: 50-60 µl 384-well plates: approximately 20 µl Recommended ArticlesA method is described for the routine determination of the rate of colorimetric enzyme reactions using a 96-well microtiter plate reader commonly used in. UV/Vis spectrophotometer & microplate reader with built-in cuvette port. Step 2: Launch SoftMax Pro Software. Four wash/rinse bottles are included and configurable for both 96- and 384. Running both standard spectrophotometer and microplate. Molecular Devices M5 Plate Reader Plate Calibration Form . 125 pM. Highly customizable low volume applications Molecular Devices unique SpectraDrop Wide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. No filter change with dual monochromator optics. In . Note: SoftMax Pro Software must be installed on a computer connected to the instrument. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. 5 mm and 15. FlexStation 3 Multi-Mode Microplate Reader A five-mode microplate reader with integrated fluid transfer Click “Next”, and the reader will start scanning the four corner wells; a progress indicator will appear: fter the reader finishes scanning the four corner wells, you will see a screen with an image of the upper left corner A well and a pink crosshair. The plate reader optics are tunable for. It is a UV/Vis spectrophotometer/microwell reader with built-in cuvette port and microplate drawer. Resipher 32x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 32x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 32 wells Utilizes Lucid Lab Cloud software for connection to any…. This paper describes a robust and rapid procedure for measuring chlorophyll using a microplate reader. book Page 1 Wednesday, October 12, 2011 4:56 PMBuilt around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340–850nm). Please click on the links below to view the Microplate Reader Comparison Tables. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. 1 OD at 230 nm. Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, CA 94089 USA Tel: 408. The top- and bottom-reading Gemini EM Microplate Reader measures fluorescence on a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well-scan modes. All stand-alone instrument functions can be controlled using the software. Microplate Readers. Microplate is validated by molecular devices for the FLIPR reader. #PLUS 384) Methods. In combination with the SpectraMax Mini Multi-Mode Microplate Reader, it enables increased throughput and detection of DNA as low as 2 ng/μL using just a few microliters of sample. M5. After 5, 7, 11, and 13 days. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. ***Figure 1. 4. At 5 seconds to read a 96-well endpoint assay, the EMax Microplate Reader has both the speed and performance to carry a heavy workload. Alpha Technology capability can be added to the system at any time as a user’s detection needs evolve. Fax 4200 BERTHOLD TECHNOLOGIES Lumat LB 9507 Tecan SpectraFluor Thermo Scientific Varioskan LUX LJL Biosystems Analyst AD 96. With theThe StakMax® Microplate Handling System is a powerful, compact, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers for improved process flow and higher throughput. The SpectraMax Plus 384 Microplate Reader for absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates. SpectraMax Glo Steady-Luc Reporter Assay Kit, Molecular Devices (Explorer kit #R8352 or Bulk kit #R8353) Competitor kit: Steady-Glo Luciferase Assay System, Promega (Cat# E2510) SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903) Purified luciferase, Promega (Cat# E1701) The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. Page: 1/4. Cells were loaded with 2 µM Fluo-4 for one hour. Insert the filled microplate into the drawer, matching well A1 with position A1. When the user applies a set temperature of 37°C, the top is heated to 37. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. #I2511)Introduction. accurately over a wide dynamic range. These instruments are all elegant and simple in their form and function. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. Compact and tunable absorbance microplate readers DATA SHEET. Up to 70% savings in overall assay time can be gained when using the Cell Cytotoxicity assay (Figure 1). Outer wells are aligned for proper viewing with FLIPR. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. Go to Product Details. 1 mL/well of media. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. Volume is not critical because water does not absorb light appreciably between 190 and 900 nm. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Fluorescence Microplate Reader Comparison Chart. Read absorbance on the SpectraMax microplate reader at the wavelength to be used for your assay, and average the resulting OD values for all wells to obtain the Plate. Optical absorbance and fluorescence were measured on a SpectraMax M5 Multi-Mode microplate reader (Molecular Devices, San Jose, CA). Three low volume 24-well bottom slides. (12) Variants in ASPH cause exertional heat illness and are associated with malignant hyperthermia susceptibility Nature CommunicationsDecember 1, 2022 Yukari Endo, Linda Groom, Alper Celik, Natalia. Flexibility to read a variety of plate formats. Supplier: Molecular Devices. Read one sample or up to 384 in a. ( a ) HCT116 cells expressing hyBRET-ERK were plated in 96-well plates and treated with serially diluted. Instrument settings can beAbsorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. ValitaTiter 96-well (Pack of 20). 96-well microplate: 90 sec; White light: 96-well/ 384 well microplate: <2 min. Working in microplates allows for smaller assays that use less sample and reagent and increases throughput. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. The system provides automation for up to 50 microplates for easy walkaway automation. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). Learn more about how absorbance is measured, and some key applications that utilize. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. In simplest terms, a plate reader measures and. book Page 9 Wednesday, July 7, 2010 2:09 PM. is required for optimum performance with standard 96-well and 384-well format microplates for all top-read applications. FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide Ordering Parts and Accessories To obtain parts and accessories, contact Molecular Devices Customer Service or your authorized service representative. 2 mm x 86 mm) Height: between 13. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. A polyclonal antibody specific to mouse/rat IL-22 was pre-coated onto a 96-well microplate. field-upgradeable option with one-of-a-kind brightfield cell segmentation, green and red. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. The list is a standard grouping supplied by Molecular Devices and includes some which cannot be used on this instrument; they can be deleted. By far the most compact reader on the market, the footprint of the reader is almost as small as the plate itself. Lucid Scientific. The reader can measure flat and round 96-well microplates and ensure accuracy by automatic lamp calibration before each reading. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. Programmable liquid handling allows for perfectly timed, automated biochemical and cell-based assays. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. for ValitaTiter Plus standards read on Molecular Devices readers. BSA concentration (µg/mL) Average OD. SpectraMax® Plus 384 provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Molecular devices The VersaMax Absorbance Microplate Reader costs between $5,500 and $6,600. DNA concentration (ng/mL) Average RFU. The Vmax Kinetic Microplate Reader is designed to measure any of the visual wavelengths so that you can use your substrate of choice. It also integrates with the. Molecular Devices announced today the launch of the new SpectraMax ® ABS and ABS Plus Microplate Readers that cover a wide range of absorbance-based applications such as ELISAs, microbial growth, and protein quantitation and can be easily integrated into full robotic systems. 54: 1. 4 Pages. the iD5 reader’s filter system. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. SpectraMax Plus 384Microplate is validated by molecular devices for the FLIPR reader. System setup and calibration are controlled from within SoftMax Pro Software. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. . Operates as a stand-alone reader or with input/output stackers and bottom reading optics. ValitaTiter Assays Setup Guide 2601A October 2023. Incubate the plate at 37 °C for approximately one hour. Figure 2. fluorescence microplate reader from Molecular Devices. i3x Multi-Mode Microplate Reader. microplate reader (Danaher Inc) Danaher Inc is a verified supplier Danaher Inc manufactures this product . 96, 384, 24, 12, 6 well types. With various on-board features and capabilities, the ThermoMax is. #VAL003 or VAL013) or ValitaTiter Plus (Molecular Devices cat. *Simple analysis and optimization. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. 92: 88. explore more. The purposes of the study were: 1) to determine the optimal wavelength settings for three cell lines. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. When reading at wavelengths. 96-well: 0. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. CloneMatrix. The official tips for the FlexStation® 3 Series Multi-Mode Microplate Readers system. The eight-channel system delivers both high precision and speed when reading 96- and 384-well microplates for endpoint measurements, kinetic assays, and spectral scans. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M4 Microplate Reader transforms each well in a microplate to a fixed. 0 fmol/well FITC 200 µL in 96 wells Bottom-read detection limit (signal 3X SD of baseline) 8. The SpectraMax Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Once the assay runs its course, the final absorbance readout will take place on a SpectraMax® iD5 Multi-Mode Microplate Reader. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. Plate was read with SpectraMax 190 absorbance microplate reader (Molecular Devices, San Jose, CA, USA) using the. Molecular Devices 1311 Orleans Drive Sunnyvale, California 94089 Telephone: +1-408. flat meniscus), but is approx 15 mL. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. 96-well and 384-well plates; Wavelength(s): 405, 450, 490, 560 and 600nm (Abs). The VersaMax and SpectraMax 190 read 96-well microplates. and luminescence microplate reader validation DATA SHEET. It features a modular design that allows users to add or change detection cartridges according to their needs. Protein quantitation Cross-talk < 0. Optical System: High energy xenon flash lamp. This document outlines references to 21 CFR Parts 58, 211 and 820 and EudraLex Annex 15 to assess the implementation of Molecular Devices microplate readers in regulated environments. Read time* 96 wells < 15 sec. 3% in white 96- and. for ValitaTiter Plus standards read on Molecular Devices readers. The ThermoMax performs automatic mixing before a reading and between readings to ensure reliable and precise results. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. We obtained three HEK-293 cell lines from Clontech, each stably transfected with a different fluorescent protein. automation for any lab. 03 pM. 96-well-plate-scan-time-2-colors-1-site-well. SpectraDrop High-Throughput Screening (HTS) Kit. Molecular Devices Corporation (the “Seller”) shall not be liable for any incidental or consequential damages. 500 USD. #VAL004 or VAL014) 96-well assay plate • Human IgG for use as standard (e. 0 H x 60. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. 1 pM. Read the microplate Step 1: Place the microplate in the reader. Molecular Devices' microplate readers. This page contains the. com. San Jose, California, June 5, 2018. 2. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. ) 20,000 10,000 30,000 40,000 50,000 60,000 70,000 80,000 0. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Vmax Kinetic Microplate Reader with SOFTmax® Pro from Molecular Devices, now part of MDS Analytical Technologies. for ValitaTiter Assays. ValitaTiter Plus 96-well (1 plate) VAL004. tools to expand the boundaries of their research. Since then, the microplate measurements of DNA, RNA, and proteins that this enabled have become very popular. Fluorescence Microplate Reader Comparison Chart. 5. #T-0254) Lysozyme (Sigma cat. Plate reader instructions Computer. This report demonstrates that the SPECTRAmax GEMINI-XS microplate spectrofluorometer, with its dual scanning monochromator and patented optical sensitivity, can be used to detect a relatively low number of cells expressing GFP. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. # 0310-5218T). The Gemini EM Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. It features a modular design that allows users to add or change detection cartridges according to their needs. “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and UV-visible absorbance. Comprehensive data analysis software. 2. The fluorescence intensity was captured with a SpectraMax Paradigm Multi-Mode Microplate Reader (Molecular Devices, LLC, San Jose, CA, USA) at 588 nm excitation and 633. to each well. #0310-5227) 96-well cell wash head (Molecular Devices Cat. Microplate readers use a vertical light path so the distance of the. Absorbance was read at 480 nm on the SpectraMax ABS Plus reader. Minneapolis, MN, USA), according to the manufacturer's protocol. 3:40 . Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modesThe SpectraMax® i3x Multi-Mode Microplate Reader measures spectral-based . The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. 1 sec. 030 fmol Fluorescence Polarization (FP). The system provides options for 20-, 40-,. Quantitate proteins rapidly with a 5-minute, room-temperature incubation. Related Products: Low Binding Plates. 03 pM. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. #655075 and #781075) SpectraMax i3x Multi-Mode Microplate Reader; SpectraMax Injector Cartridge *Figure 1. Accurate measurements are ensured by automaticThe SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. Here, we show that Molecular Devices microplate readers can easily and noninvasively measure fluorescent proteins in living cells. If flexibility becomes important in a growing lab supporting a variety of projects, the decision might come down to whether. 2 2600A ValitaTiter. Jon Cuccui, Brendan W. Prior to the reading of each plate, the microplate reader was “blanked” with a plate containing 0. F3/F5 Multi-Mode Microplate Readers. Six wells of PBS were used as blanks for calculating the lower limit of detection (LLD). The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Page 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. “The ABS Plus reader comes standard with our patented PathCheck® Technology which enables consistent results across a 96- or 384-well plate. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. Limit of Detection: < 20 attomol ATP per well Dynamic range > 9 orders of magnitude. 1/17 1907B Printed in USA . Our SpectraTest® ABS2, FL1, LM1,The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. We would like to show you a description here but the site won’t allow us. Whatever your needs, you’ll find a microplate reader that can meet them. Well scanning With well scanning, multiple points within each well can be read, providing a high. 2. Documentation:1. ValitaTiter 96-well (Pack of 20). Once the assay runs its course, the final absorbance readout will take place on a SpectraMax® iD5 Multi-Mode Microplate Reader. #N207) LAL Reagent Water (Lonza cat. Workflows of the MTT Cell Proliferation and Cell Cytotoxicity Assay. Formats available are strip washers, full plate washers and combination washer-dispensers. maximum-illumination-channels. Vrla used the device to quantify the concentration of a small molecule produced by bacteria. The Molecular Devices SpectraMax M2e Microplate Reader makes UV-Vis absorbance (200-1000 nm), fluorescence intensity (excitation 250-850 nm, emission 250-850 nm), chemiluminescence intensity (250-850 nm), and time-resolved fluorescence (50-1450 µsec, 200 µsec increments) measurements in 6, 12, 24, 48, 96, and 384 well microplates or in. Measure assay absorbance values quickly with minimal background noise on the SpectraMax iD5 reader. Can be integrated with Molecular Devices' StakMax® Microplate Stacker. Compare specifications and request a quote from leading suppliers. MAXline Microplate Readers User’s Manual iii Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the MAXline Microplate Reader excluding the lamp assembly are warranted for aPlus Microplate Reader, the R2 value was 0. #T-0254) • Lysozyme (Sigma cat. , Sigma cat. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. Fluorescence Microplate Reader Comparison Chart. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. 2 Pages. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. SpectraMax M5eMulti-Mode Microplate Reader (Molecular Devices cat. Microplates, also known as microtiter plates, are rectangular multi-well plates used in a variety of assays from ELISA to PCR. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. Read absorbance on the SpectraMax microplate reader at the wavelength to be used for your assay, and average the resulting OD values for all wells to obtain the Plate. About;. A. The absorbance at 412 nm was measured by a SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA) at 30 °C. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. Narrow bandwidths deliver increased accuracy and enhanced peak resolution. 92: 88. Fluorescence Microplate Readers. In addition, the iD5 reader measures TRF and FP and. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quantitation of firefly luciferase expression in mammalian cells. Limit of Detection: < 20 attomol ATP per well Dynamic range > 9 orders of magnitude. 3% in white 96- and 384-well microplates Time-Resolved Fluorescence Performance (M4, M5, M5e only) Reading capabilities Top or bottom of a microplateWide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. 3 OD at 200 nm. 1536-well: 0. Read More. Flexible for 6-well to 384-well microplates and cuvettes. EIGHT-CHANNEL OPTICS Plate reads as fast as five seconds MICROPLATES OR CUVETTES Use a 96- or 384-well microplate or a standard cuvette SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Streamline BCA-based protein quantitation on the SpectraMax iD5 reader. explore more. Description. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Pipet 100-200 µL of deionized water into each well of a clean 96-well microplate. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. Transfer of an assay to 96-well microplate technology increases throughput significantly. For more sample throughput, both readers can be easily integrated into full robotic Microplate Readers. Zhu P, Ju C, Sheng J, Du D, Wan J, Yin H, Xing Y, Li H, et al. Visible absorbance -. adapter plate in drawer of Plate Reader with well A1 in position A1 of the drawer. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Ensure ongoing compliance of your Molecular Devices microplate readers and be audit ready with comprehensive . The device was able to achieve 3σ limits of detection of ca. . “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate. M5. 00 Add to cart; Molecular Devices Tunable Microplate Reader VersaMax $ 1,495. 3. Gemini EM and SpectraMax M2 Microplate Readers By Yan Zhang, Molecular Devices Corporation, 1311 Orleans Dr. Affordable multi-mode microplate reader with . Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. • 0. Place the microplate in the drawer. validation, maintenance, and repair documentation. # 0310-5217T) 384-well cell wash head (Molecular Devices Cat. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™certified. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical. . Molecular Devices SpectraMax 384 Plus Microplate Reader. Injectors Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. Eight filter modes enable applications such as protein quantification, cell viability and ELISA. Read More. (Molecular Devices cat. It measures the optical density (OD) of samples in both 96- and 384-well. 03 pM 384-well: 0. 001 OD Read modes (EMax): Endpoint Read modes (VMax): Endpoint, kinetic Read times (96-well microplate): Endpoint read-only: 5 seconds. SpectraMax® 340PC384 Microplate Reader for. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. #I2511) • Assay diluent – may be cell culture medium. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. gemini em reader, molecular devices stakmax microplate stacker, softmax pro data analysis, spectrofluorometer, monochromator microplate reader, cell migration assays, cell viability assays, gfp, picogreen dna. Its software interface is easy to learn and use, and lets you control the instrument from almost any device –. EarlyTox Live/Dead Assay Kit (Molecular Devices cat. 46Click the Read button in the SoftMax Proplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. 05. A microplate reader as . White 96-well plate The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. It is user upgradeable and budget friendly, and comes with a brochure that provides detailed information on its features, specifications and applications. For more sample throughput, both readers can be easily integrated into full roboticPrimary considerations when choosing a microplate reader are throughput and flexibility. Reading Capabilities: cuvette or microplate. Compare this item. 384-well: 0. Step 3: Click “Settings” and a screen will appear (Figure 1). Microscopy. We demonstrate that miniaturizing the assay in 96-well half area microplates, and using the flexible liquid transfer features of the FlexStation ® 3 Multi-Mode Microplate Reader allows rapid and reliable measurement of EC 50 values for agonists and IC 50 values for antagonists. Both readers were heated to 37ºC prior to setting up . for 96- and 384-well microplates • Detect dim samples with unsurpassed sensitivity • Avoid saturation issues with nine orders of dynamic range • Enable robust performance with autorinse injectors • Validation and compliance tools SpectraMax® L Microplate . The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. Danaher Inc 96 well microplate reader 96 Well Microplate Reader, supplied by Danaher Inc, used in various techniques. ” Promega Corp.